Changes of alpha-2-macroglobulin activity in tear fluid in experimental retinal pigment epithelium atrophy of rabbits

Purpose. To assess the validity of alpha-2-macroglobulin ( 2-MG) activity and endothelin-1 (ET-1) concentration for characterization local metabolic disorders in experimental retinal pigment epithelium atrophy (RPE). Material methods . reproduce RPE atrophy, 22 New Zealand Albino rabbits were given a subretinal injection bevacizumab or saline. Tear fluid was collected before 3 months after it. In second series experiment, tear also on 3rd, 7th, 14th, 21st 28 th days injection. analyzed 2-MG using fermentation method ET-1 by immunoenzymatic method. Results injection, remained normal, while saline it was, average, twice as high initial one. showed significant increase over 1.5 times 3rd day both operated contralateral eyes. Conclusion. Subretinal had no lasting damaging effect retina, opposed to that led an tear. A transitory tears may indicate vascular tone elevation eye during this period. The study be used monitor shifts development, well post-transplantation process therapy adequacy.